Figure 5

The induced pluripotent stem cells (iPSCs-DSs) maintain pluripotency in vitro and in vivo. (A) Embryoid bodies (EBs) were formed by the iPSCs-DS and semiquantitative reverse transcription polymerase chain reaction (RT-PCR) analyses of lineage markers that represent the three germ layers. (B) Immunostaining showing expression of the three lineages markers Tuj1 (ectoderm), cTnT (mesoderm), and α-fetoprotein (endoderm) in DS-iPSC clones from three independent iPSC derivations subjected to EBs differentiation. (C) Teratomas were collected and stained with hematoxylin and eosin. The tissues originating from the three embryonic germ layers were present in the teratomas. Scale bars, 100 μm.