Figure 6

Colony forming units in lung from bromodeoxyuridine-positive cells sorted after 10 weeks of chase. Bromodeoxyuridine single-positive cells in the lung parenchyma were decreased 4.8-fold post-bleomycin, thus, the putative stem cell population appeared gradually depleted from lung upon bleomycin treatment. The 0.1 × 10⁶ cells were plated in semi-solid methyl cellulose and CFU were counted after 14 days. All three data points (pink squares) for bleomycin-treated (Bleo) mouse lungs were significantly increased compared to the control (Con) mouse (blue diamonds). *P < 0.05 was considered statistically significant. Data were pooled from three independent experiments (n = 4 or 5 per group). Mortality was less than 2%. Averaged data are presented as mean ± SEM of three experiments. Data were collected in duplicate petri dishes for each sample.