Figure 7

Cellularity in bronchoalveolar lavage fluid and lung before and after bleomycin challenge at day 7 in wildtype vs. gp91phox-/- and MMP12-gp91phox double knockout mice. Airways were lavaged and cells counted by a Z1 particle counter from Beckman Coulter. Lung lobes were excised after thorough exsanguination of the mouse by infra-orbital bleeding after ligation of the superior vena cava. Minced parenchyma was digested with dispase and the resultant cell suspension, filtered of cell debris, was stained with Hoechst dye, and cellularity assessed. *P < 0.05 compared to control, averaged from three independent experiments. FACsans and flow sorts were done in triplicate per sample. Samples were counted in duplicate tubes per sample for each data point (n = 4 to 5 per group). Mortality was less than 2%. Abbreviations: WS, saline-treated wildtype mice; WB, wildtype mice treated with bleomycin; NOXS, saline-treated gp91phox null; NOXB, bleomycin-treated gp91phox null; DKOS, saline treated MMP12- gp91phox double knockout; DKOB, bleomycin-treated MMP12- gp91phox double knockout.