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Table 1 Analyses in undifferentiated monolayer cultures of human mesenchymal stem cells (hMSCs)

From: SOX9 gene transfer via safe, stable, replication-defective recombinant adeno-associated virus vectors as a novel, powerful tool to enhance the chondrogenic potential of human mesenchymal stem cells

Assay No vector rAAV-lacZ rAAV-FLAG-hSOX9
  Day 7 Day 21 Day 7 Day 21 Day 7 Day 21
Viable cells 1,225 (50) 855 (25)a 1,250 (45) 835 (20)a 1,220 (40) 840(25)a
Viability (%) 92 (2) 53 (2)a 91 (3) 51 (2)a 93 (3) 53 (2)a
WST -1 (OD450 nm) 0.34 (0.02) 0.27 (0.02)a 0.32 (0.03) 0.24 (0.01)a 0.35 (0.03) 0.26 (0.02)a
DNA (ng/mg total proteins) 0.19 (0.02) 0.11 (0.01)a 0.21 (0.02) 0.12 (0.01)a 0.18 (0.01) 0.14 (0.01)a
  1. Values are given as mean (SD) by using a vector dose of 40 μl. aStatistically significant compared with earlier time point.