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Table 2 Analyses in chondrogenically differentiated aggregate cultures of human mesenchymal stem cells (hMSCs)

From: SOX9 gene transfer via safe, stable, replication-defective recombinant adeno-associated virus vectors as a novel, powerful tool to enhance the chondrogenic potential of human mesenchymal stem cells

Assay No vector rAAV-lacZ rAAV-FLAG-hSOX9
  Day 7 Day 21 Day 7 Day 21 Day 7 Day 21
Diameter (μm) 632 (6) 618 (4)c 630 (2) 614 (3)c 729 (4)a, b 982 (5)a, b, c
Cell density (cells/mm2) 433 (7) 424 (2)c 438 (4) 426 (1)c 437 (4) 425 (5)c
WST-1 (OD450 nm) 1.33 (0.17) 0.62 (0.12)c 1.29 (0.21) 0.67 (0.12)c 1.31 (0.15) 0.65 (0.14)c
DNA (ng/mg total proteins) 0.92 (0.02) 0.87 (0.02)c 0.90 (0.01) 0.86 (0.01)c 0.92 (0.01) 0.88 (0.02)c
Toluidine blue intensity (pixels) ND 167 (4) ND 169 (5) ND 914 (7)a, b
Proteoglycans (ng/mg total proteins) 0.61 (0.02) 0.79 (0.04)c 0.59 (0.03) 0.80 (0.02)c 3.37 (0.06)a, b 4.68 (0.07)a, b, c
Proteoglycans/DNA (ng/ng) 0.67 (0.04) 0.91 (0.03)c 0.66 (0.03) 0.93 (0.02)c 3.67 (0.04)a, b 5.32 (0.08)a, b, c
Type II collagen intensity (%) ND 51 (3) ND 49 (2) ND 85 (3)a, b
Type II collagen (pg/mg total proteins) 13 (2) 18 (3)c 14 (2) 19 (3)c 75 (4)a, b 112 (3)a, b, c
Type II collagen/DNA (pg/ng) 14 (2) 21 (2)c 15 (1) 22 (3)c 81 (4)a, b 127 (8)a, b, c
Type I collagen intensity (%) ND 44 (2) ND 42 (4) ND 6 (2)a, b
Type X collagen intensity (%) ND 45 (3) ND 46 (2) ND 8 (2)a, b
Alizarin red intensity (pixels) ND 57 (3) ND 56 (2) ND 5 (2)a, b
ALP activity (pg/mg total proteins) ND 57 (2) ND 55 (1) ND 25 (1)a, b
ALP/DNA (pg/ng) ND 66 (2) ND 64 (3) ND 28 (1)a, b
  1. Values are given as mean (SD) with a vector dose of 100 μl. ALP, alkaline phosphatase; ND, not done. Statistically significant compared with acondition without vector treatment, brAAV-lacZ, and cearlier time point.