Assay | No vector | rAAV-lacZ | rAAV-FLAG-hSOX9 | |||
---|---|---|---|---|---|---|
 | Day 7 | Day 21 | Day 7 | Day 21 | Day 7 | Day 21 |
Diameter (μm) | 632 (6) | 618 (4)c | 630 (2) | 614 (3)c | 729 (4)a, b | 982 (5)a, b, c |
Cell density (cells/mm2) | 433 (7) | 424 (2)c | 438 (4) | 426 (1)c | 437 (4) | 425 (5)c |
WST-1 (OD450 nm) | 1.33 (0.17) | 0.62 (0.12)c | 1.29 (0.21) | 0.67 (0.12)c | 1.31 (0.15) | 0.65 (0.14)c |
DNA (ng/mg total proteins) | 0.92 (0.02) | 0.87 (0.02)c | 0.90 (0.01) | 0.86 (0.01)c | 0.92 (0.01) | 0.88 (0.02)c |
Toluidine blue intensity (pixels) | ND | 167 (4) | ND | 169 (5) | ND | 914 (7)a, b |
Proteoglycans (ng/mg total proteins) | 0.61 (0.02) | 0.79 (0.04)c | 0.59 (0.03) | 0.80 (0.02)c | 3.37 (0.06)a, b | 4.68 (0.07)a, b, c |
Proteoglycans/DNA (ng/ng) | 0.67 (0.04) | 0.91 (0.03)c | 0.66 (0.03) | 0.93 (0.02)c | 3.67 (0.04)a, b | 5.32 (0.08)a, b, c |
Type II collagen intensity (%) | ND | 51 (3) | ND | 49 (2) | ND | 85 (3)a, b |
Type II collagen (pg/mg total proteins) | 13 (2) | 18 (3)c | 14 (2) | 19 (3)c | 75 (4)a, b | 112 (3)a, b, c |
Type II collagen/DNA (pg/ng) | 14 (2) | 21 (2)c | 15 (1) | 22 (3)c | 81 (4)a, b | 127 (8)a, b, c |
Type I collagen intensity (%) | ND | 44 (2) | ND | 42 (4) | ND | 6 (2)a, b |
Type X collagen intensity (%) | ND | 45 (3) | ND | 46 (2) | ND | 8 (2)a, b |
Alizarin red intensity (pixels) | ND | 57 (3) | ND | 56 (2) | ND | 5 (2)a, b |
ALP activity (pg/mg total proteins) | ND | 57 (2) | ND | 55 (1) | ND | 25 (1)a, b |
ALP/DNA (pg/ng) | ND | 66 (2) | ND | 64 (3) | ND | 28 (1)a, b |