Systematic assessment in an animal model of the angiogenic potential of different human cell sources for therapeutic revascularization

Table 2 Implantation of putative proangiogenic monocytes does not stimulate vascularization in subcutaneous sponge implants

		Vessel density (Chalkley counts)
Source	Implanted cells	Untreated	Treated	Difference	Probability level (ANOVA)	Incorporation into vessels
normal peripheral blood (donors: n = 3)	Adherent (plastic) MNC (mice: n = 11)	5.288 (0.742)	5.106 (0.590)	-0.182 (0.796)	0.475	No
	Non-adherent (plastic) MNC (mice: n = 12)	5.806 (0.844)	5.514 (0.840)	-0292 (1.116)	0.799	No
mobilized peripheral blood (donors: n = 1)	Adherent (plastic) MNC (mice: n = 4)	4.833 (0.791)	6.083 (0.647)	1.25 (0.610)	0.133	No
	Non-adherent (plastic) MNC (mice: n = 4)	6.208 (2.197)	7.583 (1.674)	1.375 (3.152)	0.692	No
normal peripheral blood (donors: n = 1)	Non-adherent (fibronectin) MNC (mice: n = 4)	2.667 (0.853)	4.333 (1.080)	1.667 (1.393)	0.318	No

Implantation of putative proangiogenic monocytes selected on the basis of mononuclear cells (MCN), which contain the cells responsible for early colony-forming units endothelial progenitor cells (CFU-EPC) by rapid (2h) adherence to plastic or non-adherence to fibronectin after 48 h [24, 25], did not significantly increase new vessel formation in subcutaneous sponges (P > 0.05 for all). Vessel density was not different comparing paired cell-impregnated sponges to contralateral control sponges without cells. No human cells incorporated into vessels. Data are means, with standard errors in parentheses. Probability was determined by repeat-sample analysis of variance (ANOVA) on individual readings for each mouse from pooled data from different donors.

ISSN: 1757-6512