Systematic assessment in an animal model of the angiogenic potential of different human cell sources for therapeutic revascularization

Table 3 Implantation of EOC, HUVEC or MSC does not enhance vascularization, but EOC incorporate into vessels

		Vessel density (Chalkley counts)
Source	Implanted cells	Untreated	Treated	Difference	Probability level (ANOVA)	Incorporation into vessels
Umbilical cord blood or normal peripheral blood (donors: n = 5)	Endothelial outgrowth cells (mice: n = 20)	7.528 (0.581)	9.403 (0.671)	1.875 (0.967)	0.078	Yes
Umbilical vein wall (donors: n = 2)	Human umbilical vein endothelial cells (mice: n = 7)	8.238 (1.553)	8.833 (1.492)	0.595 (1.502)	0.706	No
Outgrowth from foetal liver MNC (donors: n = 1)	Endothelial outgrowth cells) (mice: n = 4)	9.25 (1.975)	9.25 (0.308)	0.00 (2.174)	1.000	Yes
Outgrowth from bone marrow MNC *(donors:* n *= 1)*	Mesenchymal stromal cells (MSC) *(mice:* n *= 4)*	5.958 (0.893)	8.583 (1.788)	2.625 (2.260)	0.329	No
Umbilical cord blood (donors: n = 1)	EOC - extended culture (11^th passage) (mice: n = 2)	3.667 (2.000)	9.333 (2.500)	5.667 (0.500)	0.056	No
Umbilical vein wall (donors: n = 1)	HUVEC - early culture (pre first passage) (mice: n = 4)	3.958 (1.214)	5.167 (0.649)	1.208 (1.102)	0.353	Yes

Implantation of endothelial outgrowth cells (EOC), human umbilical vein endothelial cells (HUVEC) or mesechymal stromal cells (MSC) did not significantly increase new vessel formation in subcutaneous sponges (P > 0.05), as measured by the difference in vessel density between paired treated sponges containing implanted cells and contralateral control sponges without cells. Vessel density was not different comparing paired cell-impregnated sponges to contralateral control sponges without cells. EOC clearly incorporated into newly formed vessels in all sponges examined, whereas neither HUVEC nor MSC incorporated. Exceptions were late-passage EOC which did not incorporate, or very early HUVEC which did incorporate. Data are means, with standard errors in parentheses. Probability was determined by repeat-sample analysis of variance (ANOVA) on individual readings for each mouse from pooled data from different donors.

ISSN: 1757-6512