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Figure 2 | Stem Cell Research & Therapy

Figure 2

From: Human foreskin fibroblast produces interleukin-6 to support derivation and self-renewal of mouse embryonic stem cells

Figure 2

Human foreskin fibroblast supports self-renewal of mouse embryonic stem cells better than mouse embryonic fibroblast. (A) Alkaline phosphatase (AKP) staining of E14 mouse embryonic stem cells (ESCs) cultured on human foreskin fibroblast (HFF), mouse embryonic fibroblast (MEF) without exogenous leukemia inhibitory factor (LIF) or MEF with LIF for one, two and three passages, respectively. Scale bars: 100 μm. (B) The quantitative analysis of the results in (A). AKP-positive colonies in 10 (100×) microscopic fields were counted. The number of the AKP-positive colonies on HFF is set as 1.0. **P < 0.01, n = 3.

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Stem Cell Research & Therapy

ISSN: 1757-6512