Expression pluripotency markers by SCAP iPSCs in vitro. Embryonic stem cell-associated genes TRA-1-60, TRA-2-49 and OCT4 expressed by SCAP iPSCs before and after Cre-excision. Before Cre: SCAP iPSC clone 1 at passage (p)15. After Cre: SCAP iPSC-Cre clone1 at p12. Expressed genes stained in red; DAPI, nuclear stain. (scale bar: 100 μm for all staining images). DAPI, 4',6-diamidino-2-phenylindole; iPSCs, induced pluripotent stem cells; SCAP, stem cells from apical papilla.