Establishment of transgene-free induced pluripotent stem cells reprogrammed from human stem cells of apical papilla for neural differentiation

Table 1 Primers for RT-PCR.

Gene	GenBank Accession	Primer (5'--- 3')	Product size (bp)
		Forward
		Reverse
Nestin	NM_006617	AAC AGC GAC GGA GGT CTC TA TTC TCT TGT CCC GCA GAC TT	220
βIII-tubulin	NM_001197181	CAG ATG TTC GAT GCC AAG AA GGG ATC CAC TCC ACG AAG TA	164
NFM	NM_005382	CGA CCT CAG CAG CTA CCA GGA CAC CAG TGA TGC TTC CTG CAA ATG TGC T	200
NSE	NM_001975	GTC CCA CGT GTC TTC CAC TT TGG GAT CTA CAG CCA CAT GA	236
NeuN	NM_001082575	CTT ACG GAG CGG TCG TGT AT AGA AGG AAA CGG TGG AAG GT	215
GRM1	NM_000838	TGA GGG TTG TCC CTT CTG AC GGA AGC CTC TCT CGG AGT TT	241
NR1-1	NM_000832	AAG CCT CGA GGG TAC CAG AT AGC TTG ATG AGC AGG TCG AT	236
CNPase	NM_033133	GTG GAG CAC AAA AGC CTC TC AAG TTT CCC ATG TGG CTG AC	232
GFAP	NM_001131019	GCA GAT TCG AGG GGG CAA AA GAA CTC AGG GGG ATT GGG AG	221
GAPDH	NM_002046	CAA GGC TGA GAA CGG GAA GC AGG GGG CAG AGA TGA TGA CC	194

ISSN: 1757-6512