Immunophenotyping of control RCB 2080 cell line and expanded human UCB-MSCs. (A) to (F) Adherent human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) and the RCB 2080 cell line were trypsinized at passage number 3 and fluorescence-activated cell sorting (FACS) analysis was carried out with important MSC surface markers CD105 and CD29. All expanded hUCB-MSCs showed approximately 94% positivity for CD29 and 47% positivity for CD105. (G) Percentage of MSC-positive surface markers in the control RCB 2080 cell line and expanded hUCB-MSCs by FACS analysis. Blank histogram represents cells-only control. Data represented as mean ± standard deviation of triplicates (n = 3) from three different experiments.