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Figure 2 | Stem Cell Research & Therapy

Figure 2

From: Umbilical cord blood-derived mesenchymal stem cells consist of a unique population of progenitors co-expressing mesenchymal stem cell and neuronal markers capable of instantaneous neuronal differentiation

Figure 2

Isolation of human UCB-MSCs and their characterization in comparison with the human UCB-MSC cell line. (A) to (T) Immunofluorescence analysis of the control mesenchymal stem cells (MSC) cell line RCB 2080 and expanded human umbilical cord blood-derived (hUCB)-MSCs with MSC-positive surface markers CD105, CD29 and CD73 and MSC-negative surface marker CD45, clearly showing the RCB 2080 hUCB-MSC cell line as well as the expanded hUCB-MSCs staining positively for MSC-positive markers and negative for MSC-negative marker, confirming the MSC phenotype. (U) RT-PCR analysis of expanded proliferating hUCB-MSCs with MSC-positive surface markers such as CD29, CD73, CD105, and Vimentin showing good levels of expression at the mRNA level, also including the control hUCB-MSC cell line RCB 2080.

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