Neural progenitor percentage present in isolated human UCB-MSCs varies in different human UCB samples. (A) to (P) Immunofluorescence analysis with neural progenitor marker nestin and proliferation marker 5-bromo 2-deoxyuridine (BrdU) suggested the presence of proliferating neural progenitors in human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) and the number of nestin+ve/BrdU+ve cells was different from batches to batches. (Q) Percentage of nestin+ve/BrdU+ve cells in control hUCB-MSC cell line RCB 2080 and other expanded hUCB-MSCs SCB1, SCB4 and SCB10. The control RCB 2080 cell line showed only about 2% nestin+ve/BrdU+ve cells, which was significantly low when compared with the expanded hUCB-MSCs. hUCB-MSC SCB4 showed a significantly high percentage of nestin+ve/BrdU+ve cells (~23%), suggesting that these cells are more inclined to neuronal lineage. Another two batches, hUCB-MSC SCB1 and SCB10, also contained nestin+ve/BrdU+ve cells but the percentage was less compared with the hUCB-MSC SCB4, and at the same time comparatively higher than the control hUCB-MSC cell line RCB 2080. Data represented as mean ± standard deviation of triplicates (n = 3) from three different experiments. Scale bar = 100 μm. BF, Bright Field.