Neuronal differentiation of human UCB-MSCs in one-step neuronal induction medium. (A) Schematic of one-step neuronal induction condition for human umbilical cord blood-derived mesenchymal stem cell (hUCB-MSC) differentiation. After one-step neuronal induction, hUCB-MSC SCB4 generated more β-III tubulin+ve neuronal-like cells (F) to (I) compared with the control MSC cell line RCB 2080 (B) to (E). (J) to (M) β-III tubulin+ve cells observed in the case of hUCB-MSC SCB10 but less compared with hUCB-MSC SCB4, indicating a variation in differentiation potential among hUCB-MSC samples. (N) Comparison of percentage of β-III tubulin-positive cells in control RCB 2080 with hUCB-MSC SCB4 and hUCB-MSC SCB10. Only ~4% β-III tubulin-positive cells were in RCB 2080, whereas ~30% cells in hUCB-MSC SCB4 and 9.37% cells in hUCB-MSC SCB10 were positive for β-III tubulin. Data are expressed as mean ± standard deviation of triplicates (n = 3) from three different experiments. Scale bar = 100 μm. BF, Bright Field; bFGF, basic fibroblast growth factor; DAPI, 4',6-diamidino-2-phenylindole; IMDM, Iscove's modified Dulbecco's medium.