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Table 3 Polymerase chain reaction primers used to investigate osteoblastic cell differentiation

From: A new platelet cryoprecipitate glue promoting bone formation after ectopic mesenchymal stromal cell-loaded biomaterial implantation in nude mice

Gene

Identification

Forward primer

Reverse primer

Alkaline phosphatase

NM_000478

GGCCCTACAATGCTCATGTC

TGGTGGTCTTGGAGTGAGTG

Collagen type I, alpha 1

NM_000088

GAATGGAGATGATGGGGAAG

CCATCCAAACCACTGAAACC

Osteocalcin (Bglap)

NM_199173

GACTGTGACGAGTTGGCTGA

GCAAGGGGAAGAGGAAAGAA

Osteonectin (Sparc)

NM_003118

GGGCTTCTCCTCCTCTGTCT

AACCGATTCACCAACTCCAC

Osteopontin (Spp1)

NM_001040058

GCCGAGGTGATAGTGTGGTT

CATTCAACTCCTCGCTTTCC

Runx 2

NM_001024630

GCACTGGGTCATGTGTTTGA

GGCTGCATTGAAAAGACTGC

β-Actin

NM_001101

TCGTGCGTGACATTAAGGAG

AGGAAGGAAGGCTGGAAGAG

β-2-microglobulin

NM_004048

TGTCTTTCAGCAAGGACTGG

CCTCCATGATGCTGCTTACA

Gapdh

NM_002046

CCAGGTGGTCTCCTCTGACT

GGTGGTCCAGGGGTCTTACT

Hprt1

NM_000194

GACCAGTCAACAGGGGACAT

CTTGCGACCTTGACCATCTT

  1. Gapdh, glyceraldehyde-3-phosphate dehydrogenase; hprt1, hypoxanthine phosphoribosyltransferase 1.