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Table 4 Enzyme-linked immunosorbent assay quantitation of growth factor, cytokine, and chemokine levels in PGCAP, PPP, PL, TC, and FCS

From: A new platelet cryoprecipitate glue promoting bone formation after ectopic mesenchymal stromal cell-loaded biomaterial implantation in nude mice

  

EGF

FGF-b

HGF

IGF-1

PDGF-AB

TGF-b

VEGF

SDF-1a

IL-1a

IL-1b

Picograms per 1 mg protein, mean ± SEM

PGCAP

289.87 ± 50.14

5.36 ± 0.62

21.24 ± 2.73

609.61 ± 81.92

7,249.57 ± 1,305.81

2,166.77 ± 248.38

99.55 ± 26

142.88 ± 7.87

0.07 ± 0.01

2.60 ± 0.99

 

PPP

79.36 ± 7.93

1.76 ± 0.16

12.19 ± 1.1

3,310.22 ± 362.1

2,146.71 ± 237.89

15.66 ± 1.81

16.50 ± 2.08

25.10 ± 2.34

0.19 ± 0.03

0.35 ± 0.20

 

PL

44.48 ± 2.09

2.22 ± 0.91

18.76 ± 1.76

2,622.15 ± 279.86

705.42 ± 84.94

1,180.52 ± 37.61

17.09 ± 3.08

36.67 ± 5.46

0.10 ± 0.02

0.23 ± 0.05

 

TC

N.D.

0.04

0.07

16.62

0.03

42.91

0.13

65.22

0.23

0.30

 

FCS

N.D.

0.11

N.D.

1,916.29 ± 27.95

N.D.

100.03

N.D.

37.60 ± 1.22

0.57 ± 0.32

0.07

Picograms for 100 μL of products

PGCAP

2,683.15 ± 325.20

50.26 ± 6.45

189.57 ± 19.91

5,341.28 ± 395.90

63,806.01 ± 5,642.06

21,407.23 ± 2,732.49

962.80 ± 83.56

926.25 ± 84.40

0.41 ± 0.08

24.89 ± 12.83

 

TC

n.d.

0.50

0.84

191.08

0.31

493.50

1.50

150.00

0.53

0.68

Ratio

PGCAP/ PPP

3.65a

3.04b

1.74c

0.18 N.S.

3.38d

138.36a

6.03a

5.69b

0.38 N.S.

7.37c

 

PGCAP/ PL

6.52a

2.41b

1.13 N.S.

0.23 N.S.

10.28d

1.84a

5.83a

3.9b

0.71 N.S.

11.23 N.S.

 

PGACP/TC

N.D.

123.31c

292.51 N.S.

36.68 N.D.

269,806.08a

50.49d

763.24a

2.19a

0.31 N.S.

8.77 N.S.

 

PGCAP/FCS

N.D.

50.93c

N.D.

0.32c

N.D.

21.66d

N.D.

3.8a

0.12c

36.24 N.S.

 

PPP/PGCAP

0.27 N.S.

0.33 N.S.

0.57 N.S.

5.43d

0.3 N.S.

0.01 N.S.

0.17 N.S.

0.17 N.S.

2.62 N.S.

0.14 N.S.

 

PL/PGCAP

0.15 N.S.

0.41 N.S.

0.88 N.S.

4.3d

0.1 N.S.

0.54 N.S.

0.17 N.S.

0.26 N.S.

1.41 N.S.

0.09 N.S.

  1. Analysis-of-variance test followed by Student Newman-Keuls test. aP <0.001, bP <0.01, cP <0.05, dP <0.0001. EGF, epidermal growth factor; FCS, fetal calf serum; FGF-b, fibroblast growth factor-beta; HGF, hepatocyte growth factor; IGF-1, insulin-like growth factor-1; IL, interleukin; N.D., not determined; N.S., not significant; PDGF-AB, platelet-derived growth factor-AB; PGCAP, platelet glue obtained from cryoprecipitation of apheresis platelet products; PL, platelet lysate; PPP, platelet-poor plasma; SDF-1a, stromal cell-derived factor 1 alpha; SEM, standard error of the mean; TC, Tissucol; TGF-b, transforming growth factor-beta; VEGF, vascular endothelial growth factor.