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Table 4 Enzyme-linked immunosorbent assay quantitation of growth factor, cytokine, and chemokine levels in PGCAP, PPP, PL, TC, and FCS

From: A new platelet cryoprecipitate glue promoting bone formation after ectopic mesenchymal stromal cell-loaded biomaterial implantation in nude mice

   EGF FGF-b HGF IGF-1 PDGF-AB TGF-b VEGF SDF-1a IL-1a IL-1b
Picograms per 1 mg protein, mean ± SEM PGCAP 289.87 ± 50.14 5.36 ± 0.62 21.24 ± 2.73 609.61 ± 81.92 7,249.57 ± 1,305.81 2,166.77 ± 248.38 99.55 ± 26 142.88 ± 7.87 0.07 ± 0.01 2.60 ± 0.99
  PPP 79.36 ± 7.93 1.76 ± 0.16 12.19 ± 1.1 3,310.22 ± 362.1 2,146.71 ± 237.89 15.66 ± 1.81 16.50 ± 2.08 25.10 ± 2.34 0.19 ± 0.03 0.35 ± 0.20
  PL 44.48 ± 2.09 2.22 ± 0.91 18.76 ± 1.76 2,622.15 ± 279.86 705.42 ± 84.94 1,180.52 ± 37.61 17.09 ± 3.08 36.67 ± 5.46 0.10 ± 0.02 0.23 ± 0.05
  TC N.D. 0.04 0.07 16.62 0.03 42.91 0.13 65.22 0.23 0.30
  FCS N.D. 0.11 N.D. 1,916.29 ± 27.95 N.D. 100.03 N.D. 37.60 ± 1.22 0.57 ± 0.32 0.07
Picograms for 100 μL of products PGCAP 2,683.15 ± 325.20 50.26 ± 6.45 189.57 ± 19.91 5,341.28 ± 395.90 63,806.01 ± 5,642.06 21,407.23 ± 2,732.49 962.80 ± 83.56 926.25 ± 84.40 0.41 ± 0.08 24.89 ± 12.83
  TC n.d. 0.50 0.84 191.08 0.31 493.50 1.50 150.00 0.53 0.68
Ratio PGCAP/ PPP 3.65a 3.04b 1.74c 0.18 N.S. 3.38d 138.36a 6.03a 5.69b 0.38 N.S. 7.37c
  PGCAP/ PL 6.52a 2.41b 1.13 N.S. 0.23 N.S. 10.28d 1.84a 5.83a 3.9b 0.71 N.S. 11.23 N.S.
  PGACP/TC N.D. 123.31c 292.51 N.S. 36.68 N.D. 269,806.08a 50.49d 763.24a 2.19a 0.31 N.S. 8.77 N.S.
  PGCAP/FCS N.D. 50.93c N.D. 0.32c N.D. 21.66d N.D. 3.8a 0.12c 36.24 N.S.
  PPP/PGCAP 0.27 N.S. 0.33 N.S. 0.57 N.S. 5.43d 0.3 N.S. 0.01 N.S. 0.17 N.S. 0.17 N.S. 2.62 N.S. 0.14 N.S.
  PL/PGCAP 0.15 N.S. 0.41 N.S. 0.88 N.S. 4.3d 0.1 N.S. 0.54 N.S. 0.17 N.S. 0.26 N.S. 1.41 N.S. 0.09 N.S.
  1. Analysis-of-variance test followed by Student Newman-Keuls test. aP <0.001, bP <0.01, cP <0.05, dP <0.0001. EGF, epidermal growth factor; FCS, fetal calf serum; FGF-b, fibroblast growth factor-beta; HGF, hepatocyte growth factor; IGF-1, insulin-like growth factor-1; IL, interleukin; N.D., not determined; N.S., not significant; PDGF-AB, platelet-derived growth factor-AB; PGCAP, platelet glue obtained from cryoprecipitation of apheresis platelet products; PL, platelet lysate; PPP, platelet-poor plasma; SDF-1a, stromal cell-derived factor 1 alpha; SEM, standard error of the mean; TC, Tissucol; TGF-b, transforming growth factor-beta; VEGF, vascular endothelial growth factor.