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Figure 1 | Stem Cell Research & Therapy

Figure 1

From: Neurogenic and neuro-protective potential of a novel subpopulation of peripheral blood-derived CD133+ ABCG2+CXCR4+ mesenchymal stem cells: development of autologous cell-based therapeutics for traumatic brain injury

Figure 1

Isolation of mesenchymal stem cells (MSC) and subpopulations. (A) Representative dot plot for the sorting gate used in collection of CD133+ABCG2+CXCR4+ MSC. (B) Phase contrast micrograph of freshly isolated CD133+ABCG2+CXCR4+ MSC prior to, as well as (C) 24 hours post trans-retinoic acid (RA) priming. (D-F) A representative set of histograms showing flow cytometry evaluation of nestin and microtubule associated protein (MAP)-1β expression by unprimed or RA-primed CD133+ABCG2+CXCR4+ MSC from one donor; 10,000 cells were collected for each flow cytometry sample. (G-I) Representative confocal images of CD133+ABCG2+CXCR4+ MSC showing expression of nestin and MAP-1β at 24 hours. (G) Unprimed MSC. (H and I) RA-primed MSC; (H) sample stained for expression of CXCR4 (red) and nestin (green); (I) cells showing expression of CXCR4 (red) and MAP-1β (green). (G) H and I staining control. (J) MSC subpopulations were elutriated or sorted by fluorescence-activated cell sorting (FACS) based on co-expression of CD133 and CXCR4, CD133 and ABCG2 and CXCR4, or CD133 and ABCG2 and SSEA-4 and CXCR4. Data are for MSC subpopulations isolated from 10 blood donors. Results represent the average +/- SD. Statistically significant comparisons (*P < 0.05, **P < 0.005) for nestin and tyrosine hydroxylase (TH) production for elutriated but unsorted cells compared to each subpopulation of FACS-sorted MSC are shown.

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