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Figure 2 | Stem Cell Research & Therapy

Figure 2

From: Neurogenic and neuro-protective potential of a novel subpopulation of peripheral blood-derived CD133+ ABCG2+CXCR4+ mesenchymal stem cells: development of autologous cell-based therapeutics for traumatic brain injury

Figure 2

MSC subpopulation isolation and induction of neural markers. (A) Number of cells per μl isolated from peripheral blood (PB) for the CD133 and CXCR4, CD133 and ABCG2 and CXCR4, or CD133 and ABCG2 and SSEA-4 and CXCR4 subpopulations. Data are from 10 PB donors. Results represent the average +/- SD. Statistically significant comparisons (*P < 0.05, **P < 0.005) for the CD133 and CXCR4, or CD133 and ABCG2 and CXCR4 or CD133 and ABCG2 and SSEA-4 and CXCR4 subpopulations. (B) Representative histograms showing evaluation of CXCR4+CD133+ ABCG2+ MSC differentiation for cells isolated from one donor, pre-priming as well as 24 hours or 7 days post-RA priming; (B) isotype staining control, (C) expression of nestin, (D) expression of TH: 10,000 cells were collected for each sample. (E) Immunoprecipitation of newly produced 35S methionine-labeled nestin or tyrosine hydroxylase (Ty Hyd) from cells isolated from two donors (A and B); MW, molecular weight. (F) Evaluation of viability of primed or unprimed MSC isolated from 10 donors after 24 hours, 3 days (D3), or 7 (D7) days of culture.

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Stem Cell Research & Therapy

ISSN: 1757-6512