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Figure 3 | Stem Cell Research & Therapy

Figure 3

From: Neurogenic and neuro-protective potential of a novel subpopulation of peripheral blood-derived CD133+ ABCG2+CXCR4+ mesenchymal stem cells: development of autologous cell-based therapeutics for traumatic brain injury

Figure 3

Induction of neural markers in vitro. Representative confocal images of unprimed and trans-retinoic acid (RA)-primed fluorescence-activated cell sorting (FACS)-sorted CD133+ABCG2+CXCR4+ MSC showing expression of (A) nestin (red), (B) glial fibrillary acidic protein (GFAP) (green), (C) nestin (green), neuronal specific class III β-tubulin (Tuj1) (red) and neuron specific nuclear protein (NEUN) (teal nuclei, white arrow), (D)Tuj1 (green). For RA-primed MSC anticholine acetyl transferase (CHAT) and galactocerebroside (GalC) levels were similar to unprimed MSC. Diamidino-2-phenylindole (DAPI) nuclear stain (blue) was used in all preparations. (G) Efficiency of neuronal lineage marker expression as evaluated by flow cytometry of RA-primed MSC (solid black bars) after 7 days of culture to evaluate nestin, GFAP, CHAT, Tuj1, TH, GalC and NEUN by CD133+ABCG2+CXCR4+ MSC isolated from 10 donors, and cultured in neural differentiation media. Results represent the average +/- SD. Statistically significant comparisons indicated by brackets (*P < 0.05, **P < 0.005).

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Stem Cell Research & Therapy

ISSN: 1757-6512