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Figure 5 | Stem Cell Research & Therapy

Figure 5

From: Neurogenic and neuro-protective potential of a novel subpopulation of peripheral blood-derived CD133+ ABCG2+CXCR4+ mesenchymal stem cells: development of autologous cell-based therapeutics for traumatic brain injury

Figure 5

Migration capacity of mesenchymal stem cells (MSC). (A) Evaluation of the migration ability of CD133+ABCG2+CXCR4+ MSC. Results are presented as a chemotactic index (the ratio of cells moving towards stromal cell-derived factor-1 (SDF-1) or tissue homogenates compared to the number of cells moving towards media alone). Untreated (UNTR.), dexamethasone-treated (DEX TR.) and CXCR4 antibody-treated (ANTI-CXCR4 TR.) Trans-retinoic acid (RA)-primed MSC were evaluated for their ability to migrate. Migration of this cell population was also evaluated in response to uninjured (Uninj.) or injured (TBI) brain homogenates (Br. Homog.) alone or brain homogenate after treatment of MSC with anti-CXCR4 blocking antibody. Results for the migration of elutriated but unsorted MSC with and without treatment with anti-CXCR4 blocking antibody are also shown. Results represent the average +/- SD in the chemotactic index for 10 blood donors. Statistically significant comparisons are indicated in brackets (*P < 0.05, **P < 0.005). (B) Evaluation of CXCR4 expression before (green histogram) and after dexamethasone treatment of MSC (red histogram) using quantitative flow cytometry. (C) Schematic showing both the injury site (black arrow) and general regions evaluated in both uninjured and TBI rats shown in D-I (red circle). (D, E, F) Production of SDF-1 in (D) uninjured rat, (E) TBI rat at 2 days and (F) at 1 month post injury. Arrow points to positive staining for SDF-1 in a micro-vessel in the brain 2 days after transplantation of MSC. (G) Schematic showing both the injury site (black arrow) and general regions evaluated in both H and I. (H) Migration of CFSE+ CD133+ABCG2+CXCR4+ MSC. Carboxyfluorescein succinimidyl ester (CFSE)-labeled cells, green, (white arrow injury site) and (I) production of SDF-1 (red) in TBI rats as well as presence of CFSE-labeled MSC after 1 month; (D-I) 4',6-diamidino-2-phenylindole (DAPI) blue nuclei.

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