Figure 3

MSC production of NO is dependent on soluble factors from LPS-stimulated BV2 cells, and increases with LPS priming. Unprimed (A, B) and LPS-primed (C, D) MSCs were seeded at densities corresponding to coculture ratios and treated with supernatant (SN) from unstimulated (A, C) and LPS-stimulated (B, D) BV2 cultures. Values are expressed as mean ± SD of triplicate wells and from a representative of two independent experiments. *P < 0.05 compared with respective MSC controls (for D, values were compared with corresponding values in C). ^#P < 0.05 compared with corresponding values from unprimed MSCs. LPS, lipopolysaccharide; MSC, mesenchymal stem cell; NO, nitric oxide; SD, standard deviation.