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Figure 4 | Stem Cell Research & Therapy

Figure 4

From: Reciprocal interactions of mouse bone marrow-derived mesenchymal stem cells and BV2 microglia after lipopolysaccharide stimulation

Figure 4

IL-6 increases and TNF-α decreases in MSC/BV2 cocultures. A, C: IL-6 and TNF-α levels in culture supernatants of BV2 (resting and activated) and MSCs (unprimed and primed) were measured at 24 and 48 hours by using the BD Cytometric Bead Array. Values are expressed in concentration (pg/ml) and from a representative of three independent experiments. B, D: BV2 and MSCs were cocultured at ratios indicated below the graph, with or without transwell inserts. Lipopolysaccharide (LPS; 1 μg/ml) was then added to cocultures, and supernatants assayed at 24 and 48 hours by using the BD Cytometric Bead Array. Controls are LPS-treated BV2 cells. Values are fold change compared with control, defined as 1 arbitrary unit and from three independent experiments. Error bars indicate SD. *P < 0.05 compared with respective controls. MSC, mesenchymal stem cell; SD, standard deviation.

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