Figure 3

Characterization of human and mouse ASC effects on inflammatory cell infiltration in the lung after LPS injury. Administration of hASCs or mASCs, when compared to HBSS, significantly decreased the (a) total cell number, (b) infiltrating neutrophil number as determined by a modified Wright-Giemsa stain, and (c) MPO activity in BALF 24 hours after LPS exposure. For (a) and (b), N = 3, 6, 6, 10, 9, and 10 for Intact, PBS + hASC, PBS + mASC, LPS + HBSS, LPS + hASC, and LPS + mASC, respectively. For (c), N = 4, 3, 5, 4, and 5 for PBS + hASC, PBS + mASC, LPS + HBSS, LPS + hASC, and LPS + mASC, respectively. Significance was defined as *, **, and *** for P <0.05, P <0.01, and P <0.001, respectively, as compared to the HBSS-treated LPS-challenged mice. ASC, adipose-derived stem cell; BALF, bronchoalveolar lavage fluid; hASCs, human adipose-derived stem cells; HBSS, Hank's balanced salt solution; LPS, lipopolysaccharide; mASCs, mouse adipose-derived stem cells; MPO, myeloperoxidase; PBS, phosphate-buffered saline.