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Figure 4 | Stem Cell Research & Therapy

Figure 4

From: Comparison of the therapeutic effects of human and mouse adipose-derived stem cells in a murine model of lipopolysaccharide-induced acute lung injury

Figure 4

Regulatory effects of hASCs and mASCs on inflammatory responses in the lung. The lung mRNA levels of various proinflammatory cytokines and chemokines 24 hours after PBS or LPS challenge were quantified using real-time RT-PCR. All levels were normalized to beta-actin and reported as fold changes compared to unchallenged and untreated mouse levels. Steady-state mRNA levels of (a) macrophage inflammatory protein 2 (MIP-2), (b) interleukin 1 alpha (IL-1α), (c) IL-1β, (d) MIP-1α, (e) tumor necrosis factor alpha (TNF-α), and (f) IL-10 are shown for PBS-or LPS-challenged mice injected with HBSS, hASCs, or mASCs. N = 2, 4, 4, 5, 4, and 5 for Intact, PBS + hASC, PBS + mASC, LPS + HBSS, LPS + hASC, and LPS + mASC, respectively. Significance was defined as *, **, and *** for P <0.05, P <0.01, and P <0.001, respectively, compared to control groups; significant differences between the LPS-challenged cell-treatment groups (LPS + hASC and LPS + mASC) were denoted by ## for P <0.01. hASCs, human adipose-derived stem cells; HBSS, Hank's balanced salt solution; LPS, lipopolysaccharide; mASCs, mouse adipose-derived stem cells; PBS, phosphate-buffered saline; RT-PCR, reverse transcription-polymerase chain reaction.

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