Figure 5
From: BAY11 enhances OCT4 synthetic mRNA expression in adult human skin cells
Microarray analysis and quantitative real-time polymerase chain reaction (QPCR) of genes upregulated by BAY11. (a) Microarray data show four putative gene targets that were identified by over 3 × fold changes in human fibroblast (HUF) cells treated with BAY11compared with untreated HUF cells over the course of 3 days of daily synthetic mRNA transfections. (b) QPCR analysis confirmed microarray results for the four genes and demonstrated that relative expression of the four genes becomes more similar to human embryonic stem cell (hESC) expression in the presence of BAY11. Asterisks indicate statistically significant increases over control cells not receiving BAY11 where P value was less than 0.05. BAY11, BAY 11-7082; DUSP4, dual-specificity phosphatase 4; SLC16A9, solute carrier family 16, member 9.