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Table 1 The medium compositions used in the study.

From: Effects of different serum conditions on osteogenic differentiation of human adipose stem cells in vitro

Medium

Composition

Maintenance medium (MM)

10% FBS-, 10% HS-, or RegES-medium

OM1

100 nM Dex, 50 µM AsA2P, 10 mM β-GP in MM

OM2

10 nM Dex, 150 µM AsA2P, 10 mM β-GP in MM

OM3

5 nM Dex, 250 µM AsA2P, 10 mM β-GP in MM

  1. Maintenance medium (MM) was culture medium with 10% FBS or 10% HS, or defined, xeno-free RegES-medium (composition defined in Additional file 1). The osteogenic media (OM) were supplemented with dexamethasone (Dex), L-ascorbic acid 2-phosphate (AsA2-P), and beta-glycerophosphate (β-GP). MM, maintenance medium; OM, osteogenic medium; FBS, fetal bovine serum; HS, human serum; Dex, dexamethasone; AsA2-P, L-ascorbic acid 2-phosphate; β-GP, beta-glycerophosphate.