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Figure 1 | Stem Cell Research & Therapy

Figure 1

From: Identification of mouse colony-forming endothelial progenitor cells for postnatal neovascularization: a novel insight highlighted by new mouse colony-forming assay

Figure 1

Murine endothelial progenitor cell colony-forming units (EPC-CFUs). (a) Representative micrographs of large-EPC-CFUs or small-EPC-CFUs cultured from bone marrow mononuclear cells (BM-MNCs) for 8 days. Large-EPC-CFUs and small-EPC-CFUs were defined according to cell morphology as spindle-shaped cells or round cells, respectively. (b-d) EPC-CFUs were identified as double-positive cells due to 1,1'-dioctadecyl-3,3,3',3-tetramethyl-indocarbocyanine perchlorate-labeled acetylated-low density lipoprotein (acLDL-DiI) uptake (red) and BS-1 lectin reactivity (green). Scale bar represents 100 μm. (e) EPC colony-forming assay in murine peripheral blood mononuclear cells (PB-MNCs) or BM-MNCs. The frequencies of large-EPC-CFUs (white columns) or small-EPC-CFUs (black columns) from PB-MNCs or BM-MNCs (7 × 105 cells) were counted after 8 days of culture. *P < 0.05, **P < 0.01 versus PB-MNC-derived EPC-CFUs. (f) Expression patterns of endothelial nitric oxide synthase (eNOS), Flk-1, and vascular endothelial (VE)-cadherin genes in large-EPCs (L) or small-EPCs (S) from PB-MNCs or BM-MNCs. Both EPC-CFU-derived cells expressed markers of endothelial cells.

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