Skip to main content
Figure 4 | Stem Cell Research & Therapy

Figure 4

From: Identification of mouse colony-forming endothelial progenitor cells for postnatal neovascularization: a novel insight highlighted by new mouse colony-forming assay

Figure 4

Development of two types of endothelial progenitor cell colony-forming units (EPC-CFUs) in response to ischemia. (a) Fluorescence-activated cell sorting (FACS) profiles of lineage-negative bone marrow cells (BM-LNneg). FACS analysis of BM-LNneg was performed by using rat IgG antibodies against mouse c-Kit and Sca-1. (b) The percentage of c-Kit+/Sca-1+ lineage negative (KSL) population of BM-LNneg. Hindlimb ischemia induced KSL population in bone marrow. *P < 0.05 versus normal mice. (c) The frequencies of large-EPC-CFUs (white columns) and small-EPC-CFUs (black columns) from peripheral blood mononuclear cells (PB-MNCs), bone marrow mononuclear cells (BM-MNCs), BM-LNneg, and BM-KSL in normal mice (N) and hindlimb ischemic mice (I). Hindlimb ischemia increased the number of large-EPC-CFUs and total EPC-CFUs differentiated from PB-MNCs and bone marrow. *P < 0.05, **P < 0.01 versus total EPC-CFUs from normal mice. #P < 0.05, ##P < 0.01 versus large-EPC-CFUs from normal mice. Hindlimb ischemia induced the differentiation of PB-MNCs and bone marrow.

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512