LPS injection causes an inflammatory focal demyelinated lesion. (a) A T2*-weighted MRI sequence was used to visualize the LPS-induced localized inflammatory response, shown as a hyperintense signal (arrow) on the right side of the corpus callosum1 week after lesioning. (b) Influx of microglia in the right corpus callosum after injection of LPS, confirmed by isolectin B4 staining (red). (c) Demyelinated lesion in the corpus callosum after LPS insult, confirmed by Luxol Blue (blue) and cresyl violet (purple) staining at 1 week. Scale bars: 1 mm (b, c).