Stem cells implanted in the brains of lesioned animals. (a) eNCSCs (top row) and BMSCs (bottom row) were stereotactically implanted 3 mm lateral to an LPS inflammatory lesion (arrows). The cells migrated toward the LPS lesion and were seen as a darkening of the right side of the corpus callosum False-colored inserts allow better visualization of the hypointense regions. (b) In coronal sections, both IO-TAT-FITC-labeled cell types were observed to have migrated from the implantation site (arrow 1) toward the LPS-lesion site (arrow 2) along the corpus callosum. Some cells were still present along the corpus callosum (arrow 3). IB4 staining (arrow; second column; red) showed that some FITC label had been taken up by microglia. GFAP staining (arrows; third column, red) showed some glial differentiation of implanted cells. Scale bars: 1 mm (b; IO-TAT-FITC), 20 μm (b; IB4, GFAP).