Figure 6

Stem cells, administered intravenously, home to an inflammatory lesion in the brain. (a) An LPS lesion was induced on the right corpus callosum, followed by intravenous administration of 50,000 IO-TAT-FITC-labelled eNCSCs (top row) or BMSCs (bottom row). Both cell types migrated to the LPS-lesion site (arrows) from the peripheral vasculature within 24 hours of administration. False-colored inserts show better the hypointense regions. (b) Both cells types migrated to the LPS lesion after intravenous injection. The IO-TAT-FITC signal demonstrated that the cells had migrated to the lesioned brain area from the peripheral vasculature 24 hours after injection (arrows; first column). Some FITC signal was detected in IB4⁺ resident macrophages/microglia (arrows; second column; red). Similarly, limited differentiation into GFAP⁺ glia was seen (arrows; third column; red). Scale bars: 1 mm (b; IO-TAT-FITC), 20 μm (b; IB4, GFAP).