Figure 2

Xenotransplantation of mesenchymal stem cells in tumor-bearing mice resulted in graft-versus-host disease and the graft-versus-tumor effect. Tumors shrank after the xenograft transplantation of mesenchymal stem cells (MSCs) into immune-deficient nude mice and immunocompetent C3H/HeN mice. These same mice showed acute liver necrosis. I: (A) Nude mice were subcutaneously injected with the cancer cell line HNE1 or co-injected with HNE1 and MSCs. (B) Representative images of hematoxylin and Eosin staining of tumor sections. When MSCs were co-injected with HNE1 cells, a well-differentiated tumor was formed that showed well-differentiated nests and cords of squamous epithelium with keratin pearls. (C) Representative views of liver biopsies. The MSC xenotransplantation resulted in liver necrosis. (D) Representative images of hematoxylin and eosin staining of liver section from the MSC xenotransplantation group and the control group. II: (A) the IVIS imaging system was used to monitor metastatic sites in the syngeneic C3H/HeN tumor model. MSC xenotransplantation into the syngeneic tumor model resulted in tumor shrinkage. (B) Representative views of liver biopsies. MSC xenotransplantation led to liver necrosis. (C) Representative images of hematoxylin and eosin staining of liver section from the MSC xenotransplantation group and the control group. PBS, phosphate-buffered saline.