Figure 3

Left-right asymmetry on micropatterned surfaces. The cells are polarized at the boundary by positioning their centrosomes (green) and Golgi apparatuses (purple) closer to each boundary than nuclei (blue), while forming chiral alignment. (a) Polarity and chirality of muscle cells on micropatterned surfaces. The leftward bias of muscle cells on appositional boundaries creates the observed counter-clockwise (CCW) cell alignment. (b) Schematic of polarity and chirality of endothelial cells on micropatterned surfaces. The rightward bias of cell migration creates the observed clockwise (CW) alignment.