Figure 2

Infused autologous bone marrow nuclear cells are retained in the lungs after endotoxin-induced acute lung injury. To differentiate infused from endogenous cells, isolated cells were surface stained with PKH. To detected engrafted cells in the lung, a sample of lung tissue was collected at the end of the experiment and frozen in Tissue-Tek solution. A. Persistent localization of PKH staining bone marrow cells (red) in the lung following endotoxemia in the swine liver-lung perfused preparation (blue = nuclei). B. To demonstrate trapping of the cells in the lung, 0.5 cc blood sample was collected every 5 minutes from the pulmonary vein and artery, and analyzed by FACS. On B, there is a quantification of the percentage of PKH+ cells collected from blood before and after the lung. FACS, fluorescence-assisted cell sorting.