Cell morphology. (A) Morphologic images of cells cultured in different conditions: FBS, HS, XF/SF CM, and XF/SF CS at time point 4 days in passage 2. The morphology of ASCs grown in XF/SF medium is more spindle-shaped and smaller than in cells grown under serum-containing medium (FBS, HS). Scale bar, 100 μm. (B) Flow-cytometric analysis confirms the morphologic characteristics observed with light microscopy. In XF/SF culture conditions (CM and CS), the cell cloud in the forward and side scatter is more uniform and contains less debris than does a cloud of cells grown in FBS or HS medium, suggesting a more homogeneous population.