Surface-marker expression of undifferentiated ASCs. Immunophenotype of cells expanded in four different culture conditions; FBS, HS, XF/SF CM, and XF/SF CS was investigated in passages 2 and 5. The data in the diagrams are presented as mean ± SD. Cells expanded under XF/SF conditions showed significantly lower expression of CD54 (ICAM-1) compared with cells expanded in serum containing medium in passage 2. Furthermore, statistically significant differences in the expression of CD54 were seen between HS medium and XF/SF conditions in passage 5. Characteristic immunophenotypes of ASCs were maintained in every culture conditions with minor differences.