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Figure 5 | Stem Cell Research & Therapy

Figure 5

From: Development of fully defined xeno-free culture system for the preparation and propagation of cell therapy-compliant human adipose stem cells

Figure 5

Multilineage differentiation potential of ASCs. Differentiation potential of ASCs cultured in four different conditions; FBS, HS, XF/SF medium with Coating Matrix Kit, or CELLstart coating was investigated toward adipogenic, osteogenic, and chondrogenic cells. Oil Red-O staining indicates the formation of intracellular lipid in cells going through adipogenic differentiation (scale bar, 100 μm); ALP staining reveals the alkaline phosphatase activity in osteogenic-differentiation cultures (scale bar, 300 μm), and Alcian blue staining recognizes the glycosaminoglycans of the cells going through chondrogenic differentiation (scale bar, 100 μm). Adipogenesis and osteogenesis was more effective in serum-containing media, whereas clearly the most intense chondrogenesis was seen in XF/SF cultures.

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