Figure 6

Gene-expression analysis. Differentiation-specific gene-expression analysis was performed after 14 days of differentiation induction, versus control medium, in different culture conditions: FBS, HS, XF/SF medium with Coating Matrix Kit, and with CELLstart coating in passages 2 and 5. The expression of PPARγ gene (A) and aP2 gene (B) demonstrate adipogenesis after 14 days of adipogenic induction. Respectively, the expression of Runx2 (C) ALP (D), and DLX5 (E) genes indicate osteogenic differentiation after 14 days of osteogenic induction. The expression of ALP and DLX5 are presented in passage 5. A significant increase in the expression of aP2 was observed in HS-supplemented induction medium compared with HS control medium, and the cells in all the other induction media in passage 2. Further, the expression of Runx2 was significantly increased in XF/SF CM induction medium as compared with FBS/HS inductions in passage 5. Moreover, the response of DLX5 to the osteogenic induction was significantly stronger in XF/SF CM-cultured cells when compared with FBS/HS conditions, as well as XF/SF CM control in passage 5. AD, adipogenic differentiation; OD, osteogenic differentiation. The data in the diagrams are presented as mean ± SD.