Adipogenic and osteogenic differentiation potential of ASCs after serum priming. Follow-up study on the differentiation of ASCs performed in five different conditions: (1) FBS, (2) XF/SF CS primed initially in FBS, (3) XF/SF CS primed initially in HS, (4) XF/SF CM primed initially in FBS, and (5) XF/SF CM primed initially in HS. Oil Red-O staining indicates the formation of intracellular lipid in cells going through adipogenic differentiation (scale bar, 100 μm); ALP staining reveals the alkaline phosphatase activity in osteogenic differentiation cultures (scale bar, 100 μm). Inset images are the paired undifferentiated negative controls. Differentiation toward adipogenic and osteogenic cells was more efficient when primed with HS- or FBS-containing medium.