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Table 3 Primer sequences of marker genes determined

From: Development of fully defined xeno-free culture system for the preparation and propagation of cell therapy-compliant human adipose stem cells

Name Primer direction Sequences Product size (bp)
hRPLP0 1 Frw 5-AAT CTC CAG GGG CAC CAT T-3 70
Rev 5-CGC TGG CTC CCA CTT TGT-3
haP2 2 Frw 5-GGTGGTGGAATGCGTCATG-3 71
Rev 5-CAACGTCCCTTGGCTTATGC-3
hPPARG 3 Frw 5-CAGTGTGAATTACAGCAAACC −3 100
Rev 5-ACAGTGTATCAGTGAAGGAAT-3
hRUNX2 4 Frw 5-CCCGTGGCCTTCAAGGT-3 76
Rev 5-CGTTACCCGCCATGACAGTA-3
hDLX5 5 Frw 5-ACCATCCGTCTCAGGAATCG-3 75
Rev 5-CCCCCGTAGGGCTGTAGTAGT-3
hALP 6 Frw 5-ATGTCATCATGTTCCTGGGAGAT-3 79
Rev 5-TGGTGGAGCTGACCCTTGAG-3
  1. 1Ribosomal protein, large, P0, (Acc. No: NM_001002); 2fatty acid-binding protein 4, (Acc. No: NM_001442); 3peroxisome proliferator-activated receptor gamma (Acc. No: NM_015869), 4runt-related transcription factor 2 (Acc. No: NM_004348); 4distal-less homeobox transcription factor 5 (Acc. No: NM_005221); 5alkaline phosphatase (Acc. No: NM_000478).