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Figure 5 | Stem Cell Research & Therapy

Figure 5

From: Increased heme-oxygenase 1 expression in mesenchymal stem cell-derived adipocytes decreases differentiation and lipid accumulation via upregulation of the canonical Wnt signaling cascade

Figure 5

Effect of heme oxygenase-1(HO-1) induction and suppression on adipogenic markers. (A) Effect of cobalt-protoporphyrin IX (CoPP), tin (stannic)-mesophorphyrin IX (SnMP) and siRNA HO-1 on lipid accumulation. Lipogenesis was measured as the relative absorbance of Oil Red O at day 14 after inducing adipogenesis as described in Materials and methods (mean ± SD, *P < 0.05 vs control, **P < 0.05 vs control, #P < 0.05 vs control). (B-H) Effect of CoPP, SnMP and siRNA HO-1 on HO-1 expression, and adipogenic marker expression in mesenchymal stem cell (MSC)-derived adipocytes. (B) Densitometry analysis of HO-1expression (*P < 0.01 vs control, **P < 0.05 vs siRNA HO-1). (C-H) Densitometry analysis of Sonic hedgehog (shh), mesoderm-specific transcript (Mest), fatty acid binding protein (aP2), Pre-adipocyte factor-1 (Pref-1), adipogenic transcription factors CCAAT/enhancer binding protein a (C/EBPα), peroxisome proliferator-activated receptor (PPAR)γ levels, respectively (*P < 0.01 vs control, **P < 0.05 vs siRNA HO-1).

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