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Figure 7 | Stem Cell Research & Therapy

Figure 7

From: Increased heme-oxygenase 1 expression in mesenchymal stem cell-derived adipocytes decreases differentiation and lipid accumulation via upregulation of the canonical Wnt signaling cascade

Figure 7

Effect of heme oxygenase-1 (HO-1) on the Wnt canonical pathway during adipogenesis. (A) Effect of inhibition of HO-1, Wnt10b and Dickkopf 1 (Dkk1) using respective siRNA on adipocyte hypertrophy. Lipogenesis was measured as the relative absorbance of Oil Red O at day 14 after inducing adipogenesis as described in Materials and methods (mean ± SD, *P < 0.05 vs vehicle, #P < 0.05 vs siRNA Wnt10b, **P < 0.05 vs siRNA HO-1, ‡P < 0.05 vs cobalt-protoporphyrin IX (CoPP), &P < 0.05 vs siRNA Dkk1). (B-C) Effect of inhibition of HO-1, Wnt10b and Dkk1 using respective siRNA on β-catenin and p- glycogen synthase kinase (GSK) levels respectively. Data are expressed as means ± SD. (*P < 0.05 vs scramble, #P < 0.05 vs siRNA Wnt10b, **P < 0.05 vs siRNA HO-1, ‡P < 0.05 vs CoPP, &P < 0.05 vs siRNA Dkk1).

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