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Figure 1 | Stem Cell Research & Therapy

Figure 1

From: Notochordal cell conditioned medium stimulates mesenchymal stem cell differentiation toward a young nucleus pulposus phenotype

Figure 1

Schematic of experimental design to produce NCCM. NP tissue from the porcine IVD was harvested and digested to obtain a mixture of large NC and small NP cells. Large NC cells were isolated from the mixture by FACS (top FACS image is combined NC/NP cells with the reference gate to select NC cells, bottom FACS image is the isolated cells from the gate (NC cells). NC cells were embedded in alginate, and cultured for four days to produce conditioned medium (NCCM).

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