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Figure 3 | Stem Cell Research & Therapy

Figure 3

From: Notochordal cell conditioned medium stimulates mesenchymal stem cell differentiation toward a young nucleus pulposus phenotype

Figure 3

Morphology and sGAG production in MSC pellet cultures treated with NCCM and TGFβ. Cultures were examined after seven days of treatment. A) Significantly greater GAG production was noted in the NCCM than TGFβ group (* indicates significance, P = 0.04). Values are expressed as average ±SEM. sGAG/DNA normalized to CM group. B) Pellets stained with alcian blue and imaged at 20× show an increase in sGAG content with NCCM treatment. (L to R: CM, TGFβ medium, NCCM) Scale bar = 200 microns. C) NCCM and TGFβ treated pellets were larger than control pellets as analyzed by gross morphology (L to R: CM, TGFβ medium, NCCM) scale bar = 1.8 mm.

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Stem Cell Research & Therapy

ISSN: 1757-6512