Laser Doppler scanning of blood flow over hind limbs on Day 14 after critical limb ischemia (CLI). A to D) Normal hind limb blood flow prior to CLI procedure. F to I) Markedly reduced blood flow on left side on Day 2 after CLI, validating the CLI model. K to N) Notably increased blood flow in CLI wild-type (WT) Fischer rats treated with GCSF (N) compared to that in other groups by Day 14 after CLI. E, J, O) Ratio of ischemic/normal blood flow (INBF) prior to (that is, Day 0) and on days 2 and 14 after CLI. J) Notably lower INBF ratio in all groups by Day 2 after CLI compared with normal condition. O) Remarkably higher INBF ratio in the WT-CLI-GCSF group than in other groups by Day 14 after CLI procedure and without significant difference among groups 1, 2 and 3. Statistical analysis by one-way ANOVA. * vs. †, P < 0.01. Symbols (*, †) indicate significant difference (at 0.05 level) by Tukey's multiple comparison procedure (n = 9 per group).