Figure 4

Distribution of CD31+ cells in ischemic skeletal muscle on Day 14 following CLI induction. A to F) Immunofluorescent staining of CD31+ cells (white arrows) in ischemic skeletal muscle on post-CLI Day 14 with nuclei being counter-stained with DAPI (blue) (400x, n = 6 per group). G) In DPP4-deficient animals, significantly lower number of CD31+ cells in those with or without GCSF treatment than in DPP4^D-NC, without difference between the former two groups. * vs. †, P < 0.001. In WT Fischer animals, notably lower cell number in WT-CLI than in WT-NC and WT-CLI-GCSF, without difference between the latter two groups. * vs. †, P < 0.0001. For comparison among the six groups, statistical analysis with one-way ANOVA. * vs. other groups, P < 0.0001. Symbols (*, †) indicate significant difference (at 0.05 level) by Tukey's multiple comparison procedure.