Distribution of von Willebrand factor (vWF)+ cells in ischemic skeletal muscle on Day 14 following CLI induction. A to F) Immunofluorescent staining of cells positive for von Willebrand factor (vWF) (white arrows), an indicator of endothelial cells, in ischemic area of each group (n = 6) on post-CLI with nuclei being counter-stained with DAPI (blue) (400x, n = 6 per group) (400x). G) In DPP4-deficient animals, significantly lower number of vWF+ cells in those with or without GCSF treatment than in DPP4D-NC, with no difference between the former two groups. * vs. †, P < 0.01. In WT Fischer rats, notably lower number of positively-stained cells in WT-CLI than in WT-NC and WT-CLI-GCSF, without difference between the latter two groups. * vs. †, P < 0.001. For comparison among the six groups, statistical analysis by one-way ANOVA. * vs. other groups, P < 0.001. Symbols (*, †) indicate significant difference (at 0.05 level) by Tukey's multiple comparison procedure.