Figure 7
Secretion profile for growth factors of human adipose-derived stem cells in three-dimensional and two-dimensional culture. Secretion profile of growth factors of human adipose-derived stem cells in both PEGMEMA–MEO2MA–PEGDA and HA hybrid hydrogel (three-dimensional (3D)) and tissue culture plastic (two-dimensional (2D)) over 7 days. Cells were seeded at 0.5×106 cells/ml in both 3D and 2D culture conditions in 48-well plates. Supernatant from both conditions was analysed using a multiplex ELISA kit for placental-derived growth factor (PlGF), vascular endothelial growth factor (VEGF) and transforming growth factor beta (TGF-β) (n =3, P <0.05). *Statistical significance.