Figure 2

Myogenic differentiation potential is reduced in Zmpste24^-/- muscle-derived stem/progenitor cells (MDSPCs). (A) RT-PCR to measure the expression of the myogenic differentiation markers, myogenin and desmin. (B) Representative images of in vitro MDSPC myogenic differentiation. MDSPC populations were grown to confluence and then switched to differentiation media. After 2 to 3 days, cells were immunostained for the terminal differentiation myogenic marker, f-MyHC (red). Scale bar, 100 μm. (C) Graph shows the myogenic differentiation (percentage of cells (DAPI, blue) expressing f-MyHC (red)) of MDSPCs isolated from Zmpste24^-/--deficient mice relative to young WT MDSPCs. Error bars indicate SD. *P < 0.001, Student's t test. Representative images are shown from three separate experiments using three independent MDSPC populations of each group.