Figure 4

Myogenic differentiation defect of Zmpste24^-/- muscle-derived stem/progenitor cells (MDSPCs) is rescued in vitro. (A) Representative images of myotube formation by Zmpste24^-/- MDSPCs, Zmpste24^-/- MDSPCs in the presence of culture media from WT MDSPCs, or cocultured with WT MDSPCs by using transwell inserts, f-MyHC (red); nuclear counterstain (DAPI; blue). Scale bar, 100 μm. (B) Plotted is the percentage of nuclei positive for f-MyHC in mononucleated (*P < 0.05; Tukey's test, relative to Zmpste24^-/- MDSPCs and WT MDSPCs), myotubes containing two to three nuclei (immature, ^#P < 0.05; Tukey's test, in comparison with Zmpste24^-/- MDSPCs), and equal to or greater than four nuclei (mature, ^§P < 0.05; Tukey's test, relative to Zmpste24^-/- MDSPCs and WT MDSPCs) in each group. Error bars, SD.